Comparison of Normal vs Mutant Zebrafish Embryos
Grade level(s):Elementary School (K-5), Grade 1, Grade 2
Topic:Zebrafish embryos, mutants
What you need:
Magic scopes, mutant fish in dishes
We will set up 4 stations with different mutants. Groups of 5 students will be assigned to each station to observe and discuss the mutations with scientists
Objective: Apply observation, description skills and teaching to your peers what you learned.
GOAL: At the end of this lesson, students should be able to describe the mutations observed, tell each other about it in a formal setting
Lesson Implementation / Outline
Introduction to mutations (10 mins) We will give a brief explanation of why mutations occur and why scientists are so interested in learning about them in model organisms. This activity will illustrate a typical day in the laboratory and what scientists do all day. Use as context cartoons where there are mutant characters.
Observation of mutants under the microscope (15 mins) We will set up 4 stations with different mutants. Groups of 5 students will be assigned to each station to observe and discuss the mutations with scientists. Mutants observed: 1. Large heart (day 2) 2. Ventricle not contracting (day 2) 3. Blistering tails (day 1 and 2) 4. Stringy heart (day 2) Each student will be responsible for collecting one piece of information about the mutant: 1. Drawing the mutant 2. Size the heart and note the eye color 3. Heart physiology 4. Tail description and stage that the mutation is visible 5. How does the mutation affect the embryo as an adult? All students within a group should share information and at some point write all data collected in their notebooks.
Student presentations (5 mins each) Students have a chance to teach the rest of the class about the mutant fish they have just learned about and get feedback. Each group presents together and each student is responsible for communicating the piece of information collected during the study.